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pet28a btkv cas9  (Integrated DNA Technologies)
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Integrated DNA Technologies pet28a btkv cas9
CRISPR editing efficiency in Bemisia tabaci
Pet28a Btkv Cas9, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet28a+btkv+cas9/Cas9+Nuclease/pmc07194319-55-28-37
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CRISPR editing efficiency in Bemisia tabaci

Journal: The CRISPR Journal

Article Title: CRISPR-Cas9-Based Genome Editing in the Silverleaf Whitefly ( Bemisia tabaci )

doi: 10.1089/crispr.2019.0067

Figure Lengend Snippet: CRISPR editing efficiency in Bemisia tabaci

Article Snippet: We identified a lysine conserved across B. tabaci sequences and a fully conserved valine residue that defined the ends of the targeting sequence (“BtKV”; Supplementary Fig. S1 ). pET28A-BtKV-Cas9 was constructed from pET28-P2C-Cas9 10 and a gblock (IDT) of BtKV-mCherry. pET28A-P2C-Cas9 was digested with restriction enzymes BamHI and SalI.

Techniques: CRISPR, Injection, Sampling

Targeting of molecular cargo to Bemisia tabaci ovaries. The BtKV peptide targets molecular cargo (mCherry-Cas9 fusion protein) into B. tabaci ovaries and is visible in dissected mature oocytes 24 h post injection (top). No signal is visible in control ovaries (bottom).

Journal: The CRISPR Journal

Article Title: CRISPR-Cas9-Based Genome Editing in the Silverleaf Whitefly ( Bemisia tabaci )

doi: 10.1089/crispr.2019.0067

Figure Lengend Snippet: Targeting of molecular cargo to Bemisia tabaci ovaries. The BtKV peptide targets molecular cargo (mCherry-Cas9 fusion protein) into B. tabaci ovaries and is visible in dissected mature oocytes 24 h post injection (top). No signal is visible in control ovaries (bottom).

Article Snippet: We identified a lysine conserved across B. tabaci sequences and a fully conserved valine residue that defined the ends of the targeting sequence (“BtKV”; Supplementary Fig. S1 ). pET28A-BtKV-Cas9 was constructed from pET28-P2C-Cas9 10 and a gblock (IDT) of BtKV-mCherry. pET28A-P2C-Cas9 was digested with restriction enzymes BamHI and SalI.

Techniques: Injection